Journal: Journal of Diabetes Research

Article Title: Renal Protection by Genetic Deletion of the Atypical Chemokine Receptor ACKR2 in Diabetic OVE Mice

doi: 10.1155/2016/5362506

Figure Lengend Snippet: Knockout of the ACKR2 gene reduces leukocyte infiltration in diabetic mice. (a) Representative images of CD45 staining, original magnification 200x. (b) Quantitative analysis of leukocyte infiltration scored as CD45 positive pixel area per visual field. Twenty-four random fields from 3 mice per group were measured. ∗ indicates p < 0.05 versus OVE. Statistical comparisons were performed by one-way ANOVA.

Article Snippet: Renal inflammatory cell infiltration was evaluated by staining sections with rat anti-mouse CD45 antibody (Angio-Proteomie, Boston, MA).

Techniques: Knock-Out, Staining

Journal: PLoS Neglected Tropical Diseases

Article Title: Filarial Excretory-Secretory Products Induce Human Monocytes to Produce Lymphangiogenic Mediators

doi: 10.1371/journal.pntd.0002893

Figure Lengend Snippet: Matrigel was injected in the presence or absence of 10/mL IL-8, IL-6 or VEGF-A in 0.5 mL. Matrigel alone was injected as a control. Matrigel was supplemented with supernatants collected from ES-stimulated PBMCs or PBMCs cultured in media as a control and injected into rats. After 9 days, Matrigel plugs were excised, sectioned and analyzed. Representative observations are presented from a single experiment using 6 rats per group. (A) Matrigel alone (control) - H&E stain. (B) Vascular response in VEGF-A plug - H&E stain. (C) Cellular response in PBMC+ES Matrigel plug – H&E stain. (D) Lymphatic vessels (green arrow) together with blood vessels (black arrow) in an IL-6-treated Matrigel plug. (E) Example of anti-vWF staining of blood vessels in PBMC+ES Matrigel plug. (F) High power of the anti-podoplanin staining in an IL-6-containing Matrigel plug at 9 days. The scale bars represent 50 microns in A, B, D and E; 100 microns in 5C; and 10 microns in 5F.

Article Snippet: Primary Ab slides were incubated for 60 min with the monoclonal mouse anti-rat podoplanin (ReliaTech/Angio-Proteomie, Boston, MA) diluted 1∶400, or the biotin-conjugated polyclonal rabbit anti-rat IgG (Novus Biologicals, Littleton, CO) diluted 1∶100 in NAD.

Techniques: Injection, Control, Cell Culture, Staining

Journal: PLoS Neglected Tropical Diseases

Article Title: Filarial Excretory-Secretory Products Induce Human Monocytes to Produce Lymphangiogenic Mediators

doi: 10.1371/journal.pntd.0002893

Figure Lengend Snippet: Quantitative assessment of the presence of podoplanin positive areas (lymphatic endothelial elements) and vWF positive areas (blood endothelial elements) in treated Matrigel plugs recovered from rats 9 days after sub-cutaneous implantation.

Article Snippet: Primary Ab slides were incubated for 60 min with the monoclonal mouse anti-rat podoplanin (ReliaTech/Angio-Proteomie, Boston, MA) diluted 1∶400, or the biotin-conjugated polyclonal rabbit anti-rat IgG (Novus Biologicals, Littleton, CO) diluted 1∶100 in NAD.

Techniques:

Journal: PLoS ONE

Article Title: Enhancement of Cell-Based Therapeutic Angiogenesis Using a Novel Type of Injectable Scaffolds of Hydroxyapatite-Polymer Nanocomposite Microspheres

doi: 10.1371/journal.pone.0035199

Figure Lengend Snippet: Tissue sections from hind limb ischemic mice (C57BL/6NCrSlc) 7 days after transplantation of NS+BMNCs were immunofluorescently stained using anti-mouse VEGF antibody (A) or anti-mouse FGF-2 antibody (B) and DAPI. BMNCs were derived from EGFP-transgenic mice. Scale bars, 100 µm. Abbreviations: NS, nano-scaffolds; LA, unmodified PLLA microspheres; BMNCs, bone marrow mononuclear cells.

Article Snippet: Rat monoclonal anti-vascular endothelial growth factor (VEGF) antibody (clone: RM0009-2G02) was purchased from Angio-Proteomie (Boston, MA).

Techniques: Transplantation Assay, Staining, Derivative Assay, Transgenic Assay